Purification and characterization of beta-mannosidases from white rot fungus Phlebia radiata.

نویسندگان

  • Monika Prendecka
  • Agnieszka Buczyńska
  • Jerzy Rogalski
چکیده

A beta-mannosidase was purified from Phlebia radiata grown in a medium containing wheat bran or galactomannan as a carbon source. Maximal activity was observed at pH 5.5 and at 50 degrees C. Highly purified isoforms of beta-mannosidase (GM-1, GM-2) isolated from media containing galactomanan and (OT-1) media with wheat bran were obtained by means of column chromatography on Q-Sepharose, SP-Sepharose and chromatofocusing on Polybuffer Exchanger PBE-94.

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عنوان ژورنال:
  • Polish journal of microbiology

دوره 56 2  شماره 

صفحات  -

تاریخ انتشار 2007